Title

Subtitle

2016 Microbiome EPI Research 

  

                    

NAME OF THE SURVEY STUDY:

EPI Microbiome Fecal Matter Survey

INVESTIGATOR:

Dr. Jan Suchodolski, MedVet, Dr Vet Med, PhD, AGAF, DACVM, Texas A&M Gastrointestinal Laboratory, College Station, Texas, USA.

OVERVIEW AND PURPOSE:

Epi4Dogs Foundation Inc will be collaborating with Dr. Suchodolski  of Texas A&M Gastrointestinal Laboratory in supplying candidates from Epi4Dogs to assist with identifying the composition of microbiota clusters in the EPI dog.  Dr. Suchodolski has recently identified a dramatic anomaly in the fecal matter of EPI dogs, vastly different from the healthy dog, and also quite different from the IBD dog too. Based on recent findings and a better understanding of the gut microbiome, our ultimate goal is to see if there is an alternative non-antibiotic way to address SID (small intestinal dysbiosis), formerly referred to as SIBO a prevalent secondary condition in the EPI dog, instead of having to prescribe antibiotics such as Tylosin Tartrate (Tylan) and/or Metronidazole (Flagyl). Our concern with these antibiotics is that much too often, after the administration of antibiotics is completed, SID returns.

However, before we embark upon new trials to ascertain whether alternative methods vs. antibiotics might be a possibility in addressing SID in the EPI dog, we need to further assess EPI dogs fecal microbiota to support and proof  Dr. Suchodolski’s findings.

Page 1 of 3 

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 EPI Microbiome Fecal Matter Survey

 

PROTOCOL:

We will be enlisting 3 different groups of confirmed EPI candidates to participate in a
non-invasive microbiome fecal-matter survey study.  The 3 groups are:

·         Confirmed EPI dogs in stable condition with no (chronic) problems with repeated SID

·         Confirmed EPI dogs with repeated (chronic) SID issues

·         Newly confirmed EPI dogs

Participation in this study will require the pet owners to report confirmed TLI test results of their EPI dogs. If Cobalamin tests were done, we ask that those results also be reported. The pet owner will then be responsible to collect twice-weekly naturally eliminated stool samples from their EPI dog, label and freeze a small portion of the collected stool samples (approximately 1 gram; equals ½ inch in size, or approximately 1 teaspoon). When storing a stool sample you will need to date it and place in a small zip lock baggie (zip lock snack bags work well) be sure it is well sealed and then put that in yet another baggie and seal that also before putting in the freezer. This will take place over the course of 2 months. Observe the stool quality and only make a notation in the record keeping if something changes, either with the stool quality or if there is any change implemented to the regular treatment protocol during the survey, for whatever reason, it must be noted in the owner’s record keeping. Stool pictures are welcomed!  At the end of the 2 months, TAMU will make arrangements to send the pet owner a pre-paid shipping label so that the pet owner can mail the frozen stool samples to TAMU gastrointestinal laboratory free of charge.  There will be no special requirements on the pet owner’s behalf to make any change to any treatment protocol the EPI pet is currently receiving.  The only expense to the pet owner will be the purchase of a small Styrofoam cooler and ice or dry ice pack for a total expense of under $10.

1.       There are no known risks to your dog in this study.

2.       Participation in this survey is voluntary and your dog (dog’s stool samples) can be withdrawn at any time, at your discretion.

3.       Any and all information collected for this survey will be confidential. The results of the analyses are subject for publication in the veterinary academic literature, though any personally identifying features will be concealed.

4.       There are no financial incentives for participation in this survey.

INSTRUCTIONS

1.       Upon agreeing to participate in this Survey Study,  please complete  the 3rd page of this consent form  and email to the President of Epi4Dogs Foundation, Inc:  [email protected]

2.       At the end of 2 months of collecting stool samples twice a week, please contact [email protected] to alert TAMU to send you the pre-paid mailing label.

3.       When sending the stool samples to TAMU, please include both the original signed copy
of ( 1) the consent form and  (2) the worksheet

 Page 2 of 3

 

 SURVEY STUDY CONSENT (please fill out everything!)

SID group your dog is in (please check one):

(    ) SID is well-regulated in this EPI dog
(    ) SID is not well-regulated in this EPI dog
(    ) This is a newly diagnosed EPI dog

Pet owner’s name:

Owner’s address:

Owner’s email & tel:

Dog’s  first name:                                                                           Dog’s age :

Other health conditions?

 

Date trial started :

Dog’s TLI score :                                         TLI test date:

Dog’s B12 score:                                         B12 test date (if available):

Is the dog on antibiotics? If so brand?:

Antibiotic current dose & dose routine:

Current diet (brand name of food or if home prepared, list portions of individual ingredients:

 List size and number of meals fed daily:

               
Pet Owner’s Name (Print):                                                                                          Date:

 

Pet Owner’s Name (Signature):                                                                                                               

 Page 3 of 3

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EPI Microbiome Fecal Matter (stools) Survey/Study
Participant EPI (Twice-Weekly) Record Keeping

SID group your dog is in (please check one):

(    ) SID is well-regulated in this EPI dog
(    ) SID is not well-regulated in this EPI dog
(    ) This is a newly diagnosed EPI dog

1.      Pet owner’s name ____________________________________________________________

2.      Owner’s address______________________________________________________________

3.      Owner’s email & tel___________________________________________________________

4.      Dog’s  first name ________________________________ Dog’s age _______________

INSTRUCTIONS

1.      Twice a week over the course of 2 months collect a teaspoon size sample of naturally eliminated stools.

2.      Seal stool sample in a zip lock baggie, and place in another zip lock baggie seal, date and freeze.

3.      At the end of 2 months, please contact [email protected] to alert TAMU for the pre-paid mailing label.

4.       Pack the 2 months of stool samples in a small Styrofoam cooler with dry ice pacs (to keep cold) and Send this worksheet and the original consent form signed and dated with the stool samples to TAMU via the pre-paid  mailing label.

On the following pages are two monthly charts for you to record observations regarding your dog’s physical health. Please start the trial by writing on the first day a full assessment of your dog’s current condition in the following 4 categories (Stools, Stomach, Appetite, Overall Health)... and then just make a notation (in any of the following days) if/when you observe a change in any of the 4 categories for better or worse, or note if there has been a change in the regular routine (treatment protocol) :

 

STOOLS

STOMACH

APPETITE

OVERALL HEALTH

color

flatulence (farting)

good appetite

acts normal

volume

stomach noises

poor

acts ill / subdued

form/texture

vomiting

 

 

frequency

bile acid

 

 

  

FIRST MONTH

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SUNDAY     

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TUESDAY  

WEDNESDAY

THURSDAY

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SECOND MONTH

Wk

SUNDAY     

MONDAY

TUESDAY  

WEDNESDAY

THURSDAY

FRIDAY

SATURDAY

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Please contact Epi4Dogs ([email protected]) when your 60-day survey is completed. Epi4Dogs will contact TAMU to send you a pre-paid label with instructions for you to properly and free of charge ship your dog’s stool samples to TAMU.